American cities are cities that can be founded by the American civilization in Civilization V and its expansions. The first city founded is the 
Capital, and the names of future cities are chosen in the order they appear on the list below.
The names listed are default names; players can change their cities' names if they choose.
| Founding Order | City Name | Notes |
|---|---|---|
| 1 | Washington | National capital city and just across the Potomac river from the Pentagon; in-game name is a shortened form of "Washington, D.C." |
| 2 | New York | Largest city in the US, home of the Statue of Liberty and Broadway (former U.S. Capital); in-game name is a shortened form of "New York City" |
| 3 | Boston | Capital and largest city in Massachusetts (home of the minutemen) |
| 4 | Philadelphia | Largest city in Pennsylvania and sixth largest in the U.S.A. (former U.S. Capital) |
| 5 | Atlanta | Capital and largest city in Georgia (home of the busiest airport in the world) |
| 6 | Chicago | Largest city in Illinois and third largest in the U.S.A. |
| 7 | Seattle | Largest city in Washington (home of Boeing: creators of the B-17 "Flying Fortress") |
| 8 | San Francisco | City in California |
| 9 | Los Angeles | Largest city in California and second largest in the U.S.A. |
| 10 | Houston | Largest city in Texas and fourth largest in the U.S.A. |
| 11 | Portland | Largest city in Oregon; also the largest city in Maine |
| 12 | St. Louis | City in Missouri |
| 13 | Miami | City in Florida |
| 14 | Buffalo | City in New York (Shared with the Iroquois, not buildable if they are in the game) |
| 15 | Detroit | Largest city in Michigan |
| 16 | New Orleans | Largest city in Louisiana |
| 17 | Baltimore | Largest city in Maryland (former U.S. Capital) |
| 18 | Denver | Capital and largest city in Colorado |
| 19 | Cincinnati | City in Ohio |
| 20 | Dallas | City in Texas and the ninth largest in the U.S.A. |
| 21 | Memphis | City in Tennessee (shared with Egypt, not buildable if they are in the game) |
| 22 | Cleveland | City in Ohio |
| 23 | Kansas City | Largest city in Missouri |
| 24 | San Diego | City in California and eighth largest in the U.S.A. |
| 25 | Richmond | Capital of Virginia |
| 26 | Las Vegas | Largest city in Nevada |
| 27 | Phoenix | Capital and largest city in Arizona, and fifth largest in the U.S.A. |
| 28 | Albuquerque | Largest city in New Mexico |
| 29 | Minneapolis | Largest city in Minnesota |
| 30 | Pittsburgh | City in Pennsylvania |
| 31 | Oakland | City in California |
| 32 | Tampa Bay | Region in Florida (the city's name is Tampa) |
| 33 | Orlando | City in Florida |
| 34 | Tacoma | City in Washington |
| 35 | Santa Fe | Capital of New Mexico |
| 36 | Olympia | Capital of Washington |
| 37 | Hunt Valley | Unincorporated community in Baltimore County, Maryland, and home of Sid Meier |
| 38 | Springfield | Capital of Illinois (34 states in the U.S. possess a city named Springfield) |
| 39 | Palo Alto | City in California |
| 40 | Centralia | City in Pennsylvania (a ghost town due to a disastrous underground coal fire in 1962 that is still burning) |
| 41 | Spokane | City in Washington |
| 42 | Jacksonville | Largest city in Florida |
| 43 | Savannah | City in Georgia |
| 44 | Charleston | Capital of West Virginia; also major city in South Carolina |
| 45 | San Antonio | City in Texas and seventh largest in the U.S.A. |
| 46 | Omaha | Largest city in Nebraska |
| 47 | Birmingham | Largest city in Alabama |
| 48 | Honolulu | Capital and largest city in Hawai'i (Shared with Polynesia, not buildable if they are in the game) |
| 49 | Anchorage | Largest city in Alaska (largest city in the U.S. by area) |
| 50 | Sacramento | Capital of California |
| 51 | Salt Lake City | Capital and largest city in Utah |
| 52 | Reno | City in Nevada |
| 53 | Boise | Capital and largest city in Idaho |
| 54 | Milwaukee | Largest city in Wisconsin |
| 55 | Santa Cruz | City in California |
| 56 | Monterey | City in California (former capital of Spanish California) |
| 57 | Santa Monica | City in California |
| 58 | Little Rock | Capital of Arkansas |
| 59 | Columbus | Capital and largest city in Ohio |
| 60 | Lebam | Small census-designated place in Washington |