Civilization IV: Colonization contains sixteen civilizations and twenty leaders:
| Civilization
|
Leader
|
Traits
|
First City
|
| England
|
King of England
|
n/a
|
London
|
| France
|
King of France
|
n/a
|
La Rochelle
|
| Netherlands
|
King of Netherlands
|
n/a
|
Amsterdam
|
| Spain
|
King of Spain
|
n/a
|
Seville
|
| New England
|
John Adams
|
Libertarian, Tolerant
|
Jamestown
|
| George Washington
|
Disciplined, Tolerant
|
| New France
|
Samuel de Champlain
|
Enterprising, Cooperative
|
Quebec
|
| Louis de Frontenac
|
Militaristic, Cooperative
|
| New Netherlands
|
Adriaen van der Donck
|
Charismatic, Mercantile
|
New Amsterdam
|
| Peter Stuyvesant
|
Industrious, Mercantile
|
| New Spain
|
Simon Bolivar
|
Determined, Conquistador
|
Isabella
|
| Jose de San Martin
|
Resourceful, Conquistador
|
| Apache
|
Mangas Coloradas
|
Impressionable, Indulgent
|
Tiis Tseba
|
| Arawak
|
Agueybana
|
Impressionable, Gracious
|
Borikén
|
| Aztec
|
Montezuma
|
Prosperous, Gracious
|
Tenochtitlán
|
| Cherokee
|
Oconostota
|
Impressionable, Gracious
|
Chota
|
| Inca
|
Huayna Capac
|
Prosperous, Mentor
|
Cuzco
|
| Iroquois
|
Logan
|
Indulgent, Mentor
|
Onodowahagh
|
| Sioux
|
Sitting Bull
|
Impressionable, Mentor
|
Isanti
|
| Tupi
|
Cunhambebe
|
Prosperous, Indulgent
|
Sy Ara
|
